Are epizoites biological indicators of a western Mediterranean striped dolphin die-off?

During a die-off of Mediterranean striped dolphins Stenella coeruleoalba in 1990-91, 82 individuals stranded on the Spanish coast were examined for crustacean ectoparasites and epizoites. Six species were detected: Syncyarnus aequus, PenneUa sp., Xenobalanus globicipitis, Conchoderma virgatum, Lepas pectinata and Lepas cf. hillii. The barnacles L. pectinata and L. cf. hillii are reported here for the first time on cetaceans; they were attached to the teeth. C. virgaturn was also found on the teeth; this is an unusual attachment on cetaceans. X. globicipitis showed a higher prevalence on the dolphins studied than on those stranded in the same months before (1980 to 1990) and after (1991 to 1993) the epizootic. An analysis of shell and pseudocapitulum sizes of the X. globicipitis infrapopulations indicated possible recent colonization. It is suggested that settlement of some epizoites might have increased due to reduced movement of the dolphins and/or impaired regenerative and immune functioning of the skin prior to death. This might account for the exceptional occurrence of Lepas spp. attached on the teeth, the unusual attachment of C. virgatum, and the higher prevalence and apparently young infrapopulations of X. globicipitis.Aznar Avendano Francisco Javier [email protected] Balbuena Diaz-Pines, Juan Antonio, [email protected] ; Raga Esteve, Juan Antonio, [email protected]

Evaluation of three methods for biomass estimation in small invertebrates, using three large disparate parasite species as model organisms

Invertebrate biomass is considered one of the main factors driving processes in ecosystems. It can be measured directly, primarily by weighing individuals, but more often indirect estimators are used. We developed two indirect and non-destructive approaches to estimate biomass of small invertebrates in a simple manner. The first one was based on clay modelling and the second one was based on image analysis implemented with open-source software. Furthermore, we tested the accuracy of the widely used geometric approximation method (third method). We applied these three different methods to three morphologically disparate model species, an acanthocephalan worm, a crustacean and a flatworm. To validate our indirect estimations and to test their accuracy, we weighed specimens of the three species and calculated their tissue densities. Additionally, we propose an uncomplicated technique to estimate thickness of individuals under a microscope, a required measurement for two of the three indirect methods tested. The indirect methods proposed in this paper provided the best approximation to direct measurements. Despite its wide use, the geometric approximation method showed the lowest accuracy. The approaches developed herein are timely because the recently increasing number of studies requiring reliable biomass estimates for small invertebrates to explain crucial processes in ecosystems

Phase-shifting interferometry based on principal component analysis

An asynchronous phase-shifting method based on principal component analysis (PCA) is presented. No restrictions about the background, modulation, and phase shifts are necessary. The presented method is very fast and needs very low computational requirements, so it can be used with very large images and/or very large image sets. The method is based on obtaining two quadrature signals by the PCA algorithm. We have applied the proposed method to simulated and experimental interferograms, obtaining satisfactory results

Analysis of the principal component algorithm in phase-shifting interferometry

We recently presented a new asynchronous demodulation method for phase-sampling interferometry. The method is based in the principal component analysis (PCA) technique. In the former work, the PCA method was derived heuristically. In this work, we present an in-depth analysis of the PCA demodulation method

PACo: A Novel Procrustes Application to Cophylogenetic Analysis

We present Procrustean Approach to Cophylogeny (PACo), a novel statistical tool to test for congruence between phylogenetic trees, or between phylogenetic distance matrices of associated taxa. Unlike previous tests, PACo evaluates the dependence of one phylogeny upon the other. This makes it especially appropriate to test the classical coevolutionary model that assumes that parasites that spend part of their life in or on their hosts track the phylogeny of their hosts. The new method does not require fully resolved phylogenies and allows for multiple host-parasite associations. PACo produces a Procrustes superimposition plot enabling a graphical assessment of the fit of the parasite phylogeny onto the host phylogeny and a goodness-of-fit statistic, whose significance is established by randomization of the host-parasite association data. The contribution of each individual host-parasite association to the global fit is measured by means of jackknife estimation of their respective squared residuals and confidence intervals associated to each host-parasite link. We carried out different simulations to evaluate the performance of PACo in terms of Type I and Type II errors with respect to two similar published tests. In most instances, PACo performed at least as well as the other tests and showed higher overall statistical power. In addition, the jackknife estimation of squared residuals enabled more elaborate validations about the nature of individual links than the ParaFitLink1 test of the program ParaFit. In order to demonstrate how it can be used in real biological situations, we applied PACo to two published studies using a script written in the public-domain statistical software R

Beyond counting species : a new way to look at biodiversity

In modern ecology, the traditional diversity indices (usually of richness, abundance, and species evenness) have been highly revealing and useful for monitoring community and ecosystem processes. However, around two decades ago, a pioneering research team noticed that these indices did not completely resolve their open questions. Thus, they suggested changing the way biodiversity was measured. At its base, this new methodology considers the distance between species (in phylogenetic or functional terms) before subsequently applying the appropriate biodiversity indices. Including phylogenetic and functional elements in the evaluation of diversity allows us to approach the concept of biodiversity in a more comprehensive way

Growth and emigration of third-stage larvae of hysterothylacium aduncum (nematoda: anisakidae) in larval herring clupea harengus

The growth and emigration of Hystherothylacium aduncum in laboratory-reared herring larvae Clupea harengus was studied. Experimental infections of 36-day-old herring larvae resulted in 126 hosts infected with 306 H. aduncum larvae. Regression analyses showed a significant worm emigration from the rectum to the head of the fish, accompanied by an increase in worm body length. The emigration was independent of worm intensity, which suggests an ontogenetic process. Some worms departed from this pattern by moving posteriorly or by penetrating into the muscle, and in 5 cases, the larvae were observed to leave living fish. This individual variation has not been observed in previous studies and might be explained by host signals related to condition or development stage. Indirect evidence suggested parasite-induced mortality in the tanks due to the emigrations because only 4 of the 126 infected fish survived 8 days postinfection; the emigration of H. aduncum affected vital organs, such as the heart and brain, and the larvae penetrating or leaving the host's tissues can cause extensive damage to the delicate herring larvae.Balbuena Diaz-Pines, Juan Antonio, [email protected]

Advanced daylighting evaluation applied to cultural heritage buildings and museums: Application to the cloister of Santa Maria El Paular

A method to evaluate the risk of using daylight in museums and cultural heritage exhibitions is presented along this study. Although daylight is an ecological and sustainable source of energy and sometimes also an intrinsic part of the artwork, the use of Natural lighting may cause damages in them due to the difficulty of controlling its variability. The developed method quantifies the damage produced to the artworks by daylight compared to artificial light taking into account the level of radiation and its spectral distribution in space and time by comparison with the damage caused by an Illuminant A (Global Risk Factor). The method, applied to the permanent paintings exhibition in the cloister of the fifteenth century of the Monastery of Santa Maria de El Paular, certifies that the control and exploitation of Natural Light should consider an optimal balance between exposure and damage

Optical inspection of liquid crystal variable retarder inhomogeneities

Liquid crystal variable retarders (LCVRs) are starting to be widely used in optical systems because of their capacity to provide a controlled variable optical retardance between two orthogonal components of incident polarized light or to introduce a known phase shifting (PS) between coherent waves, both by means of an applied voltage. Typically, the retardance or PS introduced by an LCVR is not homogeneous across the aperture. On the one hand, the LCVR glass substrates present a global bend that causes an overall variation of the retardance or PS. On the other hand, in the manufacturing process of an LCVR, there sometimes appears a set of micro-air bubbles that causes local retardance or PS inhomogeneities. In this work, we present an interferometric technique based on a Mach-Zehnder interferometer that is insensitive to vibrations and capable of inspecting and characterizing the LCVR's retardance or PS inhomogeneities. The feasibility of the proposed method is demonstrated in the experimental results, where the LCVR retardance is measured with an error of about 0:2 rad. The thickness of possible micro-air bubbles is obtained with a resolution of about 50 nm